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Identification of some bacterial strains isolated from mangrove habitat exhibiting abilities of biofilm-forming
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Zahra Zarei jeliani   , Morteza Yousefzadi , Adnan Shahdadi |
| Department of Biology, Faculty of Science, University of Qom, Qom, Iran , morteza110110@gmail.com |
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Abstract: (422 Views) |
Background and Objective: Biofouling has long been a problem for humans. Bio-fouling communities have different ecological, economic and health effects (humans, plants and marine animals). Generally, bacterial biofilm formation is the first step of the marine fulling process. This study aimed to identify bacteria capable of forming the strongest biofilms in mangroves.
Methods: In this regard, after sampling a one-day biofilm layer formed on a wooden panel, the isolates were isolated and purified using the Marin Zobel culture medium. From 12 isolates, we selected 9 isolates for molecular identification based on their ability to form bacterial biofilms using the violet crystal method. DNA was extracted using CTAB method and molecular identification was performed using 16S rRNA gene amplification.
Findings: Among isolates, 58.3% showed strong biofilm formation,16.6% showed moderate biofilm formation, and 25% showed weak biofilm formation. Based on the results, Proteobacteria with 55.5% as dominant phylum (4 genera Alteromonas, Salinimonas, Vibrio and Pseudoalteromonas), Firmicutes with 33.3% (3 genera Bacillus, Oceanobacillus and Fictibacillus), and Bacteroidetes 11/11% (genus Tenacibaculum) was identified.
Conclusion: Marine biofilms formed on inanimate and living submerged surfaces playing a key role in the establishment of larvae of Barnacles and other macrofoulers are a promising source for safe and environmentally friendly aquatic anti-fouling approaches.
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| Keywords: 16S rRNA gene, Bacterial biofilm, Biofouling, Fouling, Mangrove forest |
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Full-Text [PDF 1674 kb]
(157 Downloads)
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Type of Study: Research/ Original/ Regular Article |
Subject:
Marine Biology
Received: 2022/05/26 | Revised: 2024/08/18 | Accepted: 2024/03/5 | ePublished: 2024/08/18
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