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:: Volume 5, Issue 20 (2015) ::
joc 2015, 5(20): 87-93 Back to browse issues page
Effects of Benzo[a]pyrene and Vibrio alginolyticus on the Tissue Structure of Immune Organs in Epinephelus coioides
Nagmeh Norouzi, Negin Salamat , Gholam Reza Eskandari, Mohammad Mousavi
, salamatnegin@yahoo.com
Abstract:   (4627 Views)
In the present study, 140 Epinephlus coioides have been divided in to 7 groups including: 1. Control, 2. Group injected with coconut oil, 3. Group treated with V. alginolyticus, 4. and 5. Fish injected firstly with 20 and 200 mg/kg Benzo[a]pyrene (BaP) and bacteria after 48h, 6. and 7. Fish treated with 20 and 200 mg/kg BaP. Samples were taken from spleen and head kidney in day 1,2,4,7 and 14 of experiment and fixed in formalin buffer solution. 5 micrometer sections were prepared from tissues and were stained by hematoxylin and eosin. The tissue alterations including increase in melanomacrophage centers, bleeding, immune cell density, increase of broken RBC and bacterial colonies were observed in the spleen and head kidney of all treatments especially in days 4 and 7.
Keywords: Benzo[a]pyrene, Vibrio alginolyticus, Spleen, Head kidney, Epinephlus coioides
Full-Text [PDF 132 kb]   (764 Downloads)    
Type of Study: Research |
Received: 2015/03/16 | Accepted: 2015/03/16 | ePublished: 2015/03/16
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Norouzi N, Salamat N, Eskandari G R, Mousavi M. Effects of Benzo[a]pyrene and Vibrio alginolyticus on the Tissue Structure of Immune Organs in Epinephelus coioides. joc. 2015; 5 (20) :87-93
URL: http://joc.inio.ac.ir/article-1-672-en.html


Volume 5, Issue 20 (2015) Back to browse issues page
نشریه علمی پژوهشی اقیانوس شناسی Journal of Oceanography
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