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Effects of Benzo[a]pyrene and Vibrio alginolyticus on the Tissue Structure of Immune Organs in Epinephelus coioides
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Nagmeh Norouzi   , Negin Salamat , Gholam Reza Eskandari , Mohammad Mousavi |
| , salamatnegin@yahoo.com |
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Abstract: (11050 Views) |
| In the present study, 140 Epinephlus coioides have been divided in to 7 groups including: 1. Control, 2.
Group injected with coconut oil, 3. Group treated with V. alginolyticus, 4. and 5. Fish injected firstly with
20 and 200 mg/kg Benzo[a]pyrene (BaP) and bacteria after 48h, 6. and 7. Fish treated with 20 and 200
mg/kg BaP. Samples were taken from spleen and head kidney in day 1,2,4,7 and 14 of experiment and
fixed in formalin buffer solution. 5 micrometer sections were prepared from tissues and were stained by
hematoxylin and eosin. The tissue alterations including increase in melanomacrophage centers, bleeding,
immune cell density, increase of broken RBC and bacterial colonies were observed in the spleen and head
kidney of all treatments especially in days 4 and 7. |
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| Keywords: Benzo[a]pyrene, Vibrio alginolyticus, Spleen, Head kidney, Epinephlus coioides |
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Full-Text [PDF 132 kb]
(2089 Downloads)
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Type of Study: Research/ Original/ Regular Article |
Received: 2015/03/16 | Revised: 2015/03/16 | Accepted: 2015/03/16 | ePublished: 2015/03/16
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