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Liver Tissue Responses in Orange Spotted Grouper, Epinephelus coioides, Caused by a Short-Term Combination Treatment with benzo[a]pyrene and Vibrio alginolyticus
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Najmeh Norouzi   , Negin Salamat , Gholam Reza Eskandari , Mohammad Mousavi |
| , salamatnegin@gmail.com |
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Abstract: (10891 Views) |
| In the current study, we investigated the effects of Benzo[a]pyrene (BaP) (a five ring aromatic
hydrocarbon) in conjunction with Vibrio alginolyticus infection on the common spotted grouper,
Epinephlus coioides. The goal of our study was to elucidate the interactions between stressors in the host
organism. In this regard, 140 healthy immature orange spotted grouper (mean weight 180±7.9 gr BW and
mean length 20.8±0.2 cm) have been divided in to 7 groups including: 1- Control, 2-Groups injected with
coconut oil, 3- Group treated with V. alginolyticus, 4,5- Fish treated with 20 and 200 mg/kg BaP, 6,7- Fish
injected with V. alginolyticus with 20 and 200 mg/kg BaP, respectively. All groups were kept for 14 days
under constant laboratory conditions after bacteria injection. Sampling was carried out on days 1, 2, 4, 7,
14 of the experiment. Tissue samples from liver were taken for histological studies and fixed in formalin
buffer solution. After routine histological process, 5 micrometer sections were prepared and stained by
hematoxylin and eosin. The results showed the pathological alterations including hepatocyte vacuolation
and melanomacrophage centers in bacteria treated group, bleeding, hepatocyte vacuolation, diss space
dilation, hepatocyte hypertrophy and degeneration in BaP treated groups. Hepatocyte vacuolation,
melanomacrophage centers, diss space dilation and tissue necrosis were observed in compound groups. |
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| Keywords: Benzo[a]pyrene, Vibrio alginolyticus, Histopathology, Liver, Epinephelus coioides. |
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Full-Text [PDF 144 kb]
(2321 Downloads)
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Type of Study: Research/ Original/ Regular Article |
Received: 2014/12/27 | Revised: 2015/02/28 | Accepted: 2014/12/27 | ePublished: 2014/12/27
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