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Isolation of Collagen from Fish Waste Material- Skin, Bones and Fins of Thunnus tonggol
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Samira Hashemi Joukar   , Ehsan Kamrani , Alireza Salarzadeh , Delaram Noukhbehzare |
| , hashemisamira27@yahoo.com |
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Abstract: (13386 Views) |
| This study investigated isolation of collagen from fish waste materials- skin, bones and fins of
Thunnus tonggol. Samples were collected from Bandar Abbas factory. Sampling was done from skin,
bones and fins of T. tonggol at three treatments with three replications. Moreover, humidity, protein, fat
and ash were surveyed. Results showed that humidity and protein amount were high in skin and there were
significant differences between skin with bones and fins at protein (p< 0.05), while there were no
significant differences between skin with bones and fins at humidity (p> 0.05). The present study showed
that amino acid of Glysin was dominant at skin, bones and fins of T. tonggol. Results showed that the rate
of amino acid of hydroxiproline (collagen indicator) in skin was more than in bones and fins (p< 0.05).
This means that there are more collagen in T. tonggol skin than in bones and fins (p< 0.05). Results of the
SDS-polyacrylamide gel electrophoresis (SDS- PAGE) showed that there were not significant differences
between skin with bones and fins at a pattern of collagen (p> 0.05) and all of them had two chains of α1
and α2. |
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| Keywords: Collagen, Skin, Fins, Bone, Thunnus tonggol. |
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Full-Text [PDF 152 kb]
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Type of Study: Research/ Original/ Regular Article |
Received: 2014/06/22 | Revised: 2015/02/28 | Accepted: 2014/06/22 | ePublished: 2014/06/22
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