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The effect of temperature on production of biomass and pigments of dinoflagellate (symbiodinium sp.) in photobioreactor biofilm
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Bhrouz Zarei Darki    , Hamideh Tahmasebi Malekrodi |
| Tarbiat Modares University , zareidarki@modares.ac.ir |
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Abstract: (1192 Views) |
Background and Objectives: This research is dedicated to investigating the ecological effect of temperature on dinoflagellate biomass and pigments (Symbiodinium sp.). For this study, sea anemone was collected from the east coast of Hormuz Island. we extracted the symbiotic dinoflagellate (Symbiodinium sp.), then were cultured in different medium of ASP12, ASP8, F2-Gillard and L1 under the constant temperatures. In order to mass peridinin production on a laboratory scale, the first, it was produced as a suspension and then cellular biomass was transferred to CDs (photobioeactor) under the tempretures of 17±1, 23±1 and 29±1 °C and the light of 100 µmol.photon.m-2.s-1. in the photobioreactor biofilm. Growth rates were measured. The concentration of peridinin and chlorophyll a pigments was calculated by spectrophotometer at 645, 663 and 665 nm.
Methods: Data analysis was done using SPSS 26 and Excel 2016 software. The data normality was evaluated by Kolmogorov-Smironov test. ANOVA test, Tukey’s test and Duncan’s test were also used to compare results.
Findings: ASP12 suspension and 23±1 °C temperature were selected as the selective culture medium and optimum temperature, respectively. the amount of biomass produced by the symbiotic cell, under the different tempretures of 17±1, 23±1 and 29±1°C was observed as amount of 47.44, 24.66 and 21.55 g/m2. peridinin concentration was observed at the amount of 35.59, 11.86 and 12.67 mg/L, under the different tempretures of 17±1, 23±1 and 29±1°C, respectively. Also chlorophylla concentration was observed as amount of 29.96, 9.33 and 10.83 mg/L under the different tempretures of 17±1, 23±1 and 29±1°C, respectively. Statistical results showed a significant difference in growth rates, chlorophylla, peridinin concentration of Zooxanthellae at three different temperatures (P<0.05).
Conclusion: The results demonstrate that a biofilm-based PBR that minimizes hydrodynamic shear forces is applicable to technical-scale cultivation of dinoflagellates. |
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| Keywords: Peridinin, Cholorophyll a, Dinophyta, Temperature |
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Full-Text [PDF 1960 kb]
(326 Downloads)
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Type of Study: Research/ Original/ Regular Article |
Subject:
Marine Biology
Received: 2022/03/8 | Revised: 2023/11/20 | Accepted: 2023/09/6 | ePublished: 2023/11/18
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