Background and Objectives: Spirulina platensis is one of the most common and widespread species of cyanobacteria that has been extensively studied in various fields of the food and drug industry. Extensive tests performed on Spirulina show that this cyanobacterium is a unique source of natural bioactive substances with potential healing properties. The aim of this study was to optimize the extraction of the bioactive peptide Spirulina platensis by the enzyme alkaline protease and to investigate the anti-inflammatory properties of the extracted spirulina peptides in mice with intestinal inflammation.
Methods: For this purpose, the RSM response surface methodology determined the optimal performance of the enzyme and the extracted peptides were dried in a freeze-dried dryer to determine the molecular weight of the peptide by electrophoresis SDS page space. The resulting peptides were stored at -20°C. To evaluate the anti-inflammatory effects in 25 Balb/C mice with inflammation 4% acetic acid was induced. After ensuring inflammation of peptides with two concentrations (1.6 and 3.8 mg/kg) and omeprazole as positive control and water as negative control to Balb/C mice with intestinal ulcer, gavage and effect These treatments were evaluated on the repair of inflammation.
Findings: The results showed that the optimal conditions of enzymatic hydrolysis of alkaline protease were obtained at 60°C, 210 minutes and pH 7.5. Histological results: The presence of inflammation in the control group and in mice with peptide at a concentration of 3.8 (mg/kg) intestinal tissue, crypt and intestinal mucosa was completely regenerated during treatment. In mice treated with peptide at a concentration of 1.6(mg/kg), a relative improvement was achieved and the crypt was regenerated, but damage to the intestinal mucosa was still observed. The alkaline phosphatase enzyme is low in mice that receive the peptide, indicating an improvement in inflammation in these mice.
Conclusion: According to the results of this study, the bioactive spirulina peptides were able to treat inflammation in the intestines of mice.