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:: Volume 11, Issue 41 (2020) ::
2020, 11(41): 73-81 Back to browse issues page
phylogenic study of dinoflagellate Gyrodinium instriatum simultaneous occurrences during redtide of Gonyaulax polygramma isolated from the coastal waters of Ramin (Makoran Sea)
Gilan Attaran Fariman n
, gilan.attaran@gmail.com
Abstract:   (4167 Views)
Harmful algae bloom (HABs; Harmful Algae Blooms), which has been known as a red tide, are in fact an increase in algae species that may produce toxin and directly and indirectly damage aquatic organisms. To identify and investigate the phylogeny of dinoflagellate bloom former species which occurred in November 2017 on the southeastern coast of Iran in the Chabahar port was carried out. In this red tide, Gyrodinium instriatum species was observed with a much lower density compare to the bloom former species. The purification of specimens were carried out under laboratory conditions and the unialgal isolate of the species was incubated in culture room under 12L: 12 D condition and 25 ± 1 ºC temperature in f2 culture media. For gene sequencing, DNA was extracted and Polymerase Chain Reaction (PCR) was performed and the gene sequences were determined in the ITS gene region and then compared to the gene sequences of the same species in the Gene Bank. The molecular and phylogenic analysis indicated that the nucleotide sequence of the species with a bootstrap of 99% was most similar to the G. instriatum species. Morphological characteristics confirmed the phylogeny results. The G. instriatum species has a worldwide distribution and potentially a bloom former species, therefore, the exact identification is essential and useful.
 
Keywords: Dinoflagellate, Gyrodinium instriatum, Phylogeny, Red tide, ITS, Makoran Sea
Full-Text [PDF 772 kb]   (1410 Downloads)    
Type of Study: Applied Article | Subject: Marine Biology
Received: 2019/06/29 | Revised: 2020/11/9 | Accepted: 2020/06/6 | ePublished: 2020/09/22
References
1. Alverca, E.; Biegala, I.C.; Kennaway, G.M.; Lewis, J. & Franca, S., 2002. In situ identification and localization ofbacteria associated with Gyrodinium instriatum (Gymnodiniales, Dinophyceae) by electron and confocal microscopy. European Journal of Phycology, 37: 523-530. [DOI:10.1017/S0967026202003955]
2. Attaran-Fariman, G., 2007. Dinoflagellater and Chatonella resting stages from recent sediment of south East coast of Iran, PhD thesis, University of Tasmania, Australia, 350 pp.
3. Attaran-Fariman G, Javid P., 2013. The phylogeny of Heterocapsa sp.(Dinophyceae) isolated from the south coast of Iran during a Cochlodinium polykrikoides bloom Turk J Bot 37:778 [DOI:10.3906/bot-1206-40]
4. Attaran-Fariman G, Bolch CJ.,2012. Morphology and Phylogeny of Scrippsiella trochoidea (Dinophyceae) A Potentially Harmful Bloom Forming Species Isolated From The Sediments of Iran's South Coast Iranian Journal of Fisheries Sciences 11:252-270
5. Biegala, I.C., Kennaway, G., Alverca, E., Lennon, J., Vaulot, D. & Simon, N., 2002, Identification of bacteria associated with dinoflagellates (Dinophyceae) Alexandrium spp. using tyramide signal amplification-fluorescent in situ hybridization and confocal microscopy, Journal of Phycology 38:404-411. [DOI:10.1046/j.1529-8817.2002.01045.x]
6. Chikwililwa, C.; McCarron, P.; Waniek, J.J.; Schulz-Bull, D.E., 2019. Phylogenetic analysis and yessotoxin profiles of Gonyaulax spinifera cultures from the Benguela Current upwelling system. Harmful algae.85: 101626 [DOI:10.1016/j.hal.2019.101626]
7. Coats, D.W. & Park, M.G., 2002, Parasitism of photosynthetic dinoflagellates by three strains of Amoebophrya (Dinophyta): parasite survival, infectivity, generation time, and host specificity, Journal of Phycology 38:520-528.1 [DOI:10.1046/j.1529-8817.2002.01200.x]
8. Dale, B., 1983, Dinoflagellate resting cyst: benthic plankton in (G.A. Gryxell, ed.) Survival strategies of the algae University press, Cambridge.
9. Eschbach, E., Scharsack, J.P., John, U. & Medlin, L.K., 2001, Improved erythrocyte lysis assay in microtitre plates for sensitive detection and efficient measurement of haemolytic compounds from ichthyotoxic algae, Journal of Applied Toxicology 21:513-519. [DOI:10.1002/jat.797]
10. Freudenthal, H.D. and Lee, J.J., 1963, Glenodinium Halli n. sp. and Gyrodinium instriatumn. sp., Dinoflagellates from New York Waters, J. Protozool., 10(2), 182-189. [DOI:10.1111/j.1550-7408.1963.tb01659.x]
11. Gárate-Lizárraga, I.; Sevilla-Torres, G.; Álvarez-Añorve, M.; Aguirre-Bahena, F.; Violante-González, J.; Rojas-Herrera, A., 2013. First Record of a red tide caused by Gyrodinium instriatum (Dinophyceae: Gymnodiniales) in Bahía de Acapulco, Guerrero. Cicimar Oceanides.28(1): 43-47
12. Guiry, M.D.; Guiry, G.M., 2020. AlgaeBase. World-wide electronic publication, National University of Ireland, Galway. http://www.algaebase.org; searched on 07 June 2020
13. Hall, T.A., 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Research, Symposium Series,41: 95-98
14. Handy, S.M., Demir, E., Hutchins, D.A., Portune, K.J., Whereat, E.B., Hare, C.E., Rose, J.M., Warner, M., Farestad, M., Cary, S.C. & Coyne, K.J., 2008, Using quantitative realtime PCR to study competition and community dynamics among Delaware Inland Bays harmful algae in field and laboratory studies, Harmful Algae 7:599-613. [DOI:10.1016/j.hal.2007.12.018]
15. Husain, M., 2003, The ecology and taxonomy of marine phytoplankton of Kuwaite with particular emphasis on harmful algae species, PhD thesis University of West Minster. London.
16. Jimenez, R., 1993, Ecological factors related to Gyrodinium instriatum bloom in the inner estuary of the Gulf of Guayaquil, 257-262, In: Smayda, T.J. & Y. Shimizu (Eds.) Toxic Phytoplankton Blooms in the Sea. Elsevier, New York.
17. Kim, K.Y.; Kim, C.H., 2007. Phylogenetic relationships among diverse dinoflagellate species occurring in coastal waters off Korea inferred from large subunit ribosomal DNA sequence data. Algae.22(2): 57-67 [DOI:10.4490/ALGAE.2007.22.2.057]
18. Kojima, N. and Kobayashi, S., 1992, Motile cell-like cyst of Gyrodinium instriatum Freudenthal et Lee (Dinophyceae). Rev. Palaeobot. Palynol., 74, 239-247. [DOI:10.1016/0034-6667(92)90009-6]
19. Matsuoka, K., 1999, Eutrophication Process Recorded in Dinoflagellate Cyst Assemblages-A Case of Yokohama Port, Tokyo Bay, Japan, Sci, Total Environ., 23, 17-35. [DOI:10.1016/S0048-9697(99)00087-X]
20. Matsuoka, K., 1985, Archeopyle structure in modern gymnodinialean dinoflagellate cysts, Review of Palaeobotany and Palynology 44: 217-231. [DOI:10.1016/0034-6667(85)90017-X]
21. Moestrup, Q, Hakanen, P, Hansen. G, Daugbjerg, N, and Ellegaard, M., 2014, OnLevanderina fissa gen.&comb. nov. (Dinophyceae) (syn. Gymnodinium fissum, Gyrodinium instriatum, Gyr. uncatenum), a dinoflagellate with a very unusual sulcus, PhycologiaVolume 53 (3), 265-292. [DOI:10.2216/13-254.1]
22. Nagasoe, S., Kim, D.I., Shimasaki, Y., Oshima, Y., Yamaguchi, M. & Honjo, T., 2006, Effects of temperature, salinity and irradiance on the growth of the red tide dinoflagellates Gyrodinium instriatum Freudenthal et Lee, Harmful Algae, 5: 20-25. [DOI:10.1016/j.hal.2005.06.001]
23. Shao, P. Chen, Y.Q. Zhou, H. Yuan, J. Qu, L.H. Zhao, D. Lin, Y.SH., 2004, Genetic variability in Gymnodiniaceae ITS regions: implications for species identification and phylogenetic analysis, Marine Biology, 144: 215-224. [DOI:10.1007/s00227-003-1157-x]
24. Shikata, T., Nagasoe, S., Matsubara, T., Yamasaki, Y., Shimasaki, Y., Oshima, Y., and Honjo, T., 2007, Effects of temperature and light on cyst germination and germinated cell survival of the noxious raphidophyte Heterosigma akashiwo, Harmful Algae, 6, 700-706. [DOI:10.1016/j.hal.2007.02.008]
25. Silva, E.S. & Franca, S., 1985, The association dinoflagellate-bacteria: their ultrastructural relationship in two species of dinoflagellates, Protistologica 21:429-446.
26. Silva, E.S., 1982, Relationship between dinoflagellates and intracellular bacteria. In: Hoppe HA, Levring T (eds) Marine Algae in Pharmaceutical Science. 2. Walter de Gruyter, Berlin, pp 269-288 [DOI:10.1515/9783110837506-015]
27. Su, H.M. & Chiang, Y.M., 1991, Dinoflagellates collected from aquaculture ponds in Southern Taiwan, Japanese Journal of Phycology 39:227-238.
28. Tomas, C.R., Zimmermann, L.A & Smith, M.D., 2004, Gyrodinium instriatum. North Carolina Sea Gran, Marine Phytoplankton Identification Series. UNC-SG-00-0X.
29. Uchida, T., Matsuyama, Y., Yamaguchi, M and Honjo, T., 1996, The life cycle of Gyrodinium instriatum (Dinophyceae) in culture, Phycol. Res., 44, 119-123. [DOI:10.1111/j.1440-1835.1996.tb00040.x]
30. Uchida, T., Toda, S., Matsuyama, Y., Yamaguchi, Y., Honjo, T., 1999, Interactions between the red tide dinoflagellates Heterocapsa circularisquama and Gymnodinium mikimotoi in laboratory culture, Journal of Experimental Marine Biology and Ecology, 241, 285-299. [DOI:10.1016/S0022-0981(99)00088-X]
31. Wang, Z., Qi, Y., Yin, Y., Jiang,T., & Xie, L., 2001, Studies on the cause and the occurrence reasons of a Gyrodinium instriatum red tide in Shenzhen Bay in spring of 1998. Marine Science, 25(5):47-50.
32. Wang, Y., Yu, Z., Song, X., Zhang, Sh., 2006, Intraction between the bloom- forming dinoflagellatrs Prorocentrum donghaiense and Alexandrium Tamarense in laboratory cultures, Journal of sea Research 56 (1), 17-26. [DOI:10.1016/j.seares.2006.04.002]
33. Xu, F.L., Yang, B., Yang, C., Shuang, H.Q., Kong, X.Z., Wang, Q.M., Ouyang, H.L., Qin, N., Liu, W.X., He, W., Jiang, Y.J., 2014, The seasonal and spatial variations of phytoplankton community andtheir correlation with environmental factors in a large eutrophicChinese lake (Lake Chaohu), Ecological Indicators. 40 : 58-67. [DOI:10.1016/j.ecolind.2014.01.006]
34. Zadabbas Shahabadi, Z. Attaran Fariman G. Farahmand,H., 2011, Gyrodinium instriatum internal transcribed spacer 1, partia sequence; 5.8S ribosomal RNA gene, complete sequence; and interna transcribed spacer 2, partial sequence. https://www.ncbi.nlm. nih.gov/nuccore/JN020162.
35. Yamasaki, Y., Nagasoe, S., Matsubara, T., Shikata, T. Shimasaki, Y., Oshima, Y., Honjo, T., 2007, Growth inhibition and formation of morphologically abnormal cells of Akashiwo sanguinea (Hirasaka) 32. G. Hansen et Moestrup by cell contact with Cochlodinium polykrikoides Margalef, Marine Biology 152 (1), 157-163. [DOI:10.1007/s00227-007-0671-7]



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Attaran Fariman n G. phylogenic study of dinoflagellate Gyrodinium instriatum simultaneous occurrences during redtide of Gonyaulax polygramma isolated from the coastal waters of Ramin (Makoran Sea). Journal of Oceanography 2020; 11 (41) :73-81
URL: http://joc.inio.ac.ir/article-1-1422-en.html


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Volume 11, Issue 41 (2020) Back to browse issues page
نشریه علمی پژوهشی اقیانوس شناسی Journal of Oceanography
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