The Spatial and temporal variability of macrobenthos in the southern Caspian Sea (Chalus shore) were seasonally investigated from winter 2005 till fall 2006. Samples were taken with a Van Veen grab from depths of 7 and 20 meters along two transects, from Khate Hasht area (western part of coast) to Radio Darya area (eastern part of the coast). Totally, 5 classes of macrobenthos were identified in which the most abundant populations were Polychaeta 68.7% from abundance point of view. Density and biomass of macrobenthos in between seasons were significant (P< 0.05), as the maximum density and biomass of macrobenthos were during the summer and autumn (4251.8±1419 ind/m2 and 475±243.8 g/m2), respectively while the minimum density and biomass were during the spring and winter (725.9±64.5 ind/m2 and 0.3±0.1 (ME±SE) g/m2). A  significant correlation (P< 0/05) between the density and biomass of macrobenthos with sand percentage and total organic atter (TOM) were found in different seasons. The maximum diversity and richness (1.60±0.29 and 1.52±0.27) were during the autumn in the depth of 20 meter of Khate Hasht area, respectively while the minimum (0.85±0.27 and 0.86±0.22) were during the spring in the same depth and area. The maximum evenness (0.91±0.05) were during the winter in the depth of 7 meter of Radio Darya area and the minimum (0.55±0.13) were during the summer in the depth of 7 meter of Khate Hasht area. According to the results of ecological indices (diversity, richness and evenness) and comparison with the proposed standards, stress and pollution in Chalus shores are moderate.