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Study of Antennal Glands Role on Osmoregulation of Crayfish (Astacus leptodactylus)
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Majid Askari Hesni   , Fatemeh Lavajoo |
| , mahesni@gmail.com |
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Abstract: (8915 Views) |
| The Osmoregulation is one of the active mechanisms in crayfish that is performed by antennal glands,
digestive tract and gills. In this investigation, the effect of salinity shock on the variations of antennal
gland cells in narrow-clawed crayfish (Astacus leptodactylus) was studied. Salinity experiment treatments
were 4, 6, 8, 10 and 12gr/l with 3 replicates with control group. The specimens were gradually transferred
from freshwater to salt waters. After 72 hours, all the crayfishes were suddenly transferred from freshwater
to saltwater in order to make salinity shock. For histology studies, antennal glands samples were taken
from the crayfish before and after salinity shock and immediately fixed in Bouin solution. 5μ sections of
sectioned were prepared and stained by Hematoxylin-Eosin general staining method, then, they were
studied with Micrometer and light microscopic. In histological study of antennal glands, with increasing
the salinity, the antennal glands cells size deccreased. In contrast, after salinity shock, the size of antennal
glands cells was increased to 59.12, 62.18, 70.13, 77.66 and 79.18 μ in 4, 6, 8, 10 and 12 gr/l, respectively
and hyperplasia was clearly observed in treatment specimens. The results of statistical analysis showed
significant relationship between environment osmolarity and cells size (P< 0.05). These changes indicate
the increasing in cell osmolarity, water absorption and consequently increasing of antennal glands cells
size in each treatment after shock. The results of the study showed that variations in salinity and
osmolarity affect antennal glands cells and through the water uptake or intake body osmolarity is
regulated. |
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| Keywords: Antennal glands, Cell Changes, Salinity, Crayfish, Astacus leptodactylus. |
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Full-Text [PDF 145 kb]
(4107 Downloads)
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Type of Study: Research/ Original/ Regular Article |
Received: 2014/12/28 | Revised: 2015/02/28 | Accepted: 2014/12/28 | ePublished: 2014/12/28
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