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Determination the Lethal Concentration (LC50) of Potassium Dichromate and Behavioral Responses in Epaulet Grouper (Epinephelus stoliczkae)
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Parvin Sadeghi   , Ahmad Savari , Abdolali Movahedinia , Alireza Safahieh , Daniel Azhdari |
| , parvin.sadeghi@gmail.com |
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Abstract: (15272 Views) |
| The LC50-96h test paradigm is used to measure the susceptibility and survival potential of organisms
exposed to particular toxic substances, such as heavy metals. In this study, the Median Lethal
Concentration (LC50) of potassium dichromate (K2Cr2O7) during 96 h was determined in Epinephelus
stoliczkae. Six groups of experimental fish (7 fish in each group) with 218±0.5 g weight average and 29.6
cm total length were exposed to different concentration of dichromate potassium (0, 62, 66, 70, 74 and 78
mg/L) for 96 h. The experiments were performed in triplicate (21 fish per each treatment). The
temperature, salinity and pH were continuously measured throughout the experiments that were
27.5±0.50C, 37 ppt and 8.1, respectively. The LC50-96h was determined to be 73.09 mg/L in a static
bioassay test system. Mortality percentage increased with increasing duration of exposure and increasing
chromium concentrations. The behavioral changes observed in Epinephelus stoliczkae in the experiment
period included breathing difficulty, slow down motility, loss of balance, capsizing , swimming on water
surface, gathering around the ventilation filter, increased mucus secretion, bloodshed of gills, mouth and
fish fins and change of body coloration. Acute toxicity effect of potassium dichromate increased with
increasing its concentrations. |
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| Keywords: Chromium, LC50, Heavy metal, Epinephelus stoliczkae, Behavior |
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Full-Text [PDF 149 kb]
(3693 Downloads)
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Type of Study: Research/ Original/ Regular Article |
Received: 2014/06/20 | Revised: 2015/02/28 | Accepted: 2014/06/20 | ePublished: 2014/06/20
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