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Molecular identification of dominant species in zebra fish (Danio rerio ) and Light microscopic study of balbiani body.
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Shokouh Sadeghinia   , Solmaz Shirali  , Bita Archangi , Faedeh Amini |
| Department of Marine Biology, Faculty of Marine science, Khorramshahr University of Marine Science and Technology, Khuzestan Province, Iran , solmazshirali@kmsu.ac.ir |
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Abstract: (1395 Views) |
Background and Objectives: The primary oocyte of almost all animals contains a transitory complex called Balbiani bodies. The establishment of oocyte asymmetry is a key prerequisite for successful development across the animal kingdom, and a structure of the oocyte thought to be responsible for determining cytoplasmic polarity is the so-called Balbiani body. Due to the scarcity of published information and the great variation in appearance, ultrastructure, and functional homology between species, the molecular structure of Balbiani bodies remains a mystery.
Methods: The purpose of the present study is to accurately identify the 6 different colors of zebrafish and record its genomic information in the gene bank, as well as to examine the details of the species in the samples by optical microscopy. For this purpose, first the species were identified genetically and then the ovaries were examined histologically and histometrically after tissue passage.
Findings: The findings showed that all 6 studied samples are Danio rerio (Hamilton, 1822). Balbiani bodies were observed in all the studied samples in the previtellogenic stage.
Conclusion: Balbiani body in all samples were very similar in terms of appearance, position and size. In the oocyte of sample number 5, two Balbiani bodies were observed, with the difference that all other 5 samples contained one parietal body. |
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| Keywords: Histology, Ovary, Oogenesis, Previtellogenesis, Zebra fish, Molecular identification |
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Full-Text [PDF 1415 kb]
(276 Downloads)
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Type of Study: Research/ Original/ Regular Article |
Subject:
Marine Biology
Received: 2022/08/26 | Revised: 2024/02/29 | Accepted: 2023/06/5 | ePublished: 2023/09/10
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