:: Volume 11, Issue 41 (2020) ::
2020, 11(41): 73-81 Back to browse issues page
phylogenic study of dinoflagellate Gyrodinium instriatum simultaneous occurrences during redtide of Gonyaulax polygramma isolated from the coastal waters of Ramin (Makoran Sea)
Gilan Attaran Fariman n
, gilan.attaran@gmail.com
Abstract:   (3662 Views)
Harmful algae bloom (HABs; Harmful Algae Blooms), which has been known as a red tide, are in fact an increase in algae species that may produce toxin and directly and indirectly damage aquatic organisms. To identify and investigate the phylogeny of dinoflagellate bloom former species which occurred in November 2017 on the southeastern coast of Iran in the Chabahar port was carried out. In this red tide, Gyrodinium instriatum species was observed with a much lower density compare to the bloom former species. The purification of specimens were carried out under laboratory conditions and the unialgal isolate of the species was incubated in culture room under 12L: 12 D condition and 25 ± 1 ºC temperature in f2 culture media. For gene sequencing, DNA was extracted and Polymerase Chain Reaction (PCR) was performed and the gene sequences were determined in the ITS gene region and then compared to the gene sequences of the same species in the Gene Bank. The molecular and phylogenic analysis indicated that the nucleotide sequence of the species with a bootstrap of 99% was most similar to the G. instriatum species. Morphological characteristics confirmed the phylogeny results. The G. instriatum species has a worldwide distribution and potentially a bloom former species, therefore, the exact identification is essential and useful.
 
Keywords: Dinoflagellate, Gyrodinium instriatum, Phylogeny, Red tide, ITS, Makoran Sea
Full-Text [PDF 772 kb]   (1182 Downloads)    
Type of Study: Applied Article | Subject: Marine Biology
Received: 2019/06/29 | Revised: 2020/11/9 | Accepted: 2020/06/6 | ePublished: 2020/09/22
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Volume 11, Issue 41 (2020) Back to browse issues page