:: Volume 8, Issue 31 (2017) ::
2017, 8(31): 19-26 Back to browse issues page
Morphological and Molecular Investigation of Sargassum vulgare and Sargassum fallax (Fucales, Sargassaceae) from the Coasts of Bushehr Nuclear Power Plant, Persian Gulf
Sudabeh Iraninasab , Bita Archangi , Faedeh Amini , Nasrin Sakhai
, bita.archangi@gmail.com
Abstract:   (7194 Views)
In this research, morphological and molecular identification of dominant brown algae species from Bushehr Nuclear Power Plant coastal areas were investigated. Sampling of marine algae was carried out during autumn and winter 2014 from intertidal areas. Detailed morphological observations were done using valid identification keys and microscope. To undertake molecular identification, marine algae genome was extracted first using modified CTAB method followed by amplification of gene fragments using nuclear ribosomal ITS-2 primer. Genetic analysis was undertaken. The results of Blast in GeneBank and phylogenetic analysis confirmed identification of two species of dominant brown algae as Sargassum vulgare and Sargassum fallax with 100% bootstrap. Hence, despite taxonomic uncertainty of sargassum genus, integrating the results of morphological observations and ITS-2 gene fragment confirmed identification of both sargassum species with 100% bootstrap. 
Keywords: Brown algae, Sargassum vulgare, Sargassum fallax, ITS-2, Bushehr Nuclear Power Plant, Persian Gulf.
Full-Text [PDF 114 kb]   (1989 Downloads)    
Type of Study: Research/ Original/ Regular Article |
Received: 2017/06/6 | Accepted: 2017/11/25 | ePublished: 2018/03/7
References
1. Abbas, A.; Shameel, M., 2009. Anatomical studies on Colpomenla sinuous (Phaeophycota) Fromkarachi coast of Pakistan. Pakistan Journal of Botany, 41: 1921-1926.#
2. Abbas, A.; Shameel, M., 2014. Morpho-Anatomy of Styopodium zonale (Phaeophyta) from the coast of karachi. Pakistan Journal of Botany, 46: 1495-1499.#
3. Andrade-Sorcia, G.; Riosmena-Rodríguez, R.; Muñiz-Salazar, R.; López-Vivas, J.M.; Boo, G.H.; Lee, K.M.; Boo, S.M., 2014. Morphological reassessment and molecular assessment of Sargassum (Fucales: Phaeophyceae) species from the Gulf of California, México. Phytotaxa, 183: 201-223.#
4. Borgesen, F., 1939. Marine algae from the Persian Gulf especially from the innermost part near Bushire and the Island Kharg. In: Jessen K, Spärck R (eds) Danish Scientific Investigations in Iran, Part I. Copenhagen, 47–141PP.#
5. Bitner, L.; Payri, C.E.; Couloux, A.; Cruaud, C.; De Reviers B.; Rousseau, F., 2008. Molecular phylogeny of the Dictyotales and their position within the Phaeophyceae based on nuclear, plastid and mitochondrial DNA sequence data. Molecular Phylogenetic and Evolution Journal, 49: 211-226.#
6. Baldock, R.N., 2009. Pictured key to the southern Australian species of Sargassum Algae Revealed. Australian State Herbarium, Australia, 140P.#
7. Bast, F., 2014. Sequence similarity search, multiple sequence alignment, model selection, distance matrix and phylogeny reconstruction. Nature Protocol Exchange, 1: 1-5.#
8. Cho, G.Y.; Rousseau, F.; De Reviers, B.; Boo, S.M., 2006. Phylogenetic relationships within the Fucales (Phaeophyceae) assessed by the photosystem I coding psaA sequences. Phycologia, 45: 512-519.#
9. Coyer, A.; Hoarau, G.; Oudot–Le Secq, M.; StamW.T.; Olsen, J.L., 2006. A mtDNA – based phylogeny of the brown algal genus Fucus (Heterokontophyta; Phaeophyta). Molecular Phylogenetics and Evolution Journal, 39: 209-222.#
10. Cho, S.M.; Lee, S.M.; KO, Y.D.; Mattio, L.; Boo, S.M., 2012. Molecular systematic reassessment of Sargassum (Fucales, Phaeophyceae) in Korea using four gene regions. Botanica Marina, 55: 473-484.#
11. Camacho, O.; Mattio, L.; Draisma, S.; Fredericq, S.; Diaz- Pulido, G., 2015. Morphological and molecular assessment of Sargassum (Fucales, Phaeophyceae) from Caribbean Colombia, including the proposal of Sargassum giganteum sp. nov., Sargassum schnetteri comb. Nov and Sargassum section Cladophyllum sect. nov. Systematics and Biodiversity, 13: 105-130.#
12. Draisma, S.G.A.; Baliesteros, E.; Rousseau, F.; Thibaut, T., 2010. DNA sequence data demonstrate the polyphyly of the genus Cystoseira and other Sargassaceae genera (Phaeophyceae). Journal of Phycology, 46: 1329-1345.#
13. Dixon, R.R.M.; Huisman, J.M.; Buchanan, J.; Gurgel, C.F.D.; Spencer, P., 2012. A morphological and molecular study of austral Sargassum (Fucales, phaeophyceae) supports the recognition of phyllotricha at genus level, with further additions to the genus Sargassopsais. Journal of Phycology, 48: 1119-1129.#
14. Garretta, A., 2000. Cystoseira C. Agardh. In: A. Gomez Garreta (Editor). Flora phycologica Iberica, Vol.1 Fucales. University of Murcia, Publications Services, Barcelona, 99-101P.#
15. Guiry, M.D.; Guiry, G.M., 2017. Algae Base. Galway: World-wide electronic publication, National University of Ireland. http://www.algaebase.org, accessed 30 September 2014.#
16. Hurtado, A.Q.; Ragaza, A.R., 1999. Sargassum studies in Currimao, Ilocos Norte, Northern Philippines I. Seasonal variations in the biomass of Sargassum carpophyllum J. Agardh, Sargassum ilicifolium (Turner) C. Agardh and Sargassum siliquosum J. Agardh (Phaeophyta, Sargassaceae). Botanica Marina, 42(4), 321-325.#
17. Jegou, C.; Culoili, G.; Kervarek, N.; Simon, G.; Stiger-Pouvreau, V., 2010. LC/ESI-MS and 1H HR-MAS NMR analytical methods as useful taxonomical tools within the genus Cystoseira C. Agardh (Fucales; Phaeophyceae). Talanta, 83: 613-622.#
18. Kurihara, A.; West, J.A.; Conklin, K.Y.; Sherwood, A.R., 2012. A second species of Rhodochlya from Hawaii, with a description of R. hawaiiana cryptogamie, Algology, 33: 21-33.#
19. Mattio, L.; Payri, C.E., 2011. 190 years of Sargassum Taxonomy, Facing the Advent of DNA Phylogenies. The Botanical Review, 77: 31-70.#
20. Phillips, N.; Fredericq, S., 2000. Biogeographic and phylogenetic investigations of the pantropical genus Sargassum (Fucales, Phaeophyceae) with respect to Gulf of Mexico Species. Gulf of Mexico Science, 2: 77-87.#
21. Richmond, R.H.P.; Houk, M.; Trianni, E.; Wolanski, G.; Davis,V.; Paul, V.J., 2008. Aspects of biology and ecology functioning of coral reefs in Guam and the Commonwealth of the Northern Mariana Island: Riegl, B.M., Dodge, R.E. (eds.), coral reefs of the word. Springer, 719-739P.#
22. Sohrabipour, J.; Rabii, R., 1999. A list of marine algae of seashores of Persian Gulf and Oman Sea in the Hormozgan Province. Iranian Journal of Botany, 8: 131-162.#
23. Stiger, V.; Horiguchi, T.; Yoshida, T.; Coleman, A.W.; Masuda, M., 2003. Phylogenetic relationships inferred from ITS-2 nDNA comparisons within the genus Sargassum (Fucales, Phaeophyceae) from the Pacific basin, with an emphasis on the taxonomic subdivision of the genus. Phycological Research, 51: 1-10.#
24. Shams, M.; Afsharzadeh, S.; Balali, Gh.; De Clerck, O., 2013. Revision Sargassum species (Fucals, Phaeophyceae) from Persian Gulf and Oman Sea (Iran) based on morphological and phylogenetical analyses. Proceeding of 18th National Symposium on Applied Biological Sciences. February. 27-30, 2013. Ghent University, Ghent, Belgium, 253P.#
25. Shams, M.; Afsharzadeh, S.; Balali, Gh., 2015. Taxonomic Study of Six Sargassum Species (Sargassaceae, Fucales) with Compressed Primary Branches in the Persian Gulf and Oman Sea Including S. binderi Sonder a New Record Species for Algal Flora, Iran. Journal of Sciences, Islamic Republic of Iran, 26: 7-16.#
26. Kumar, S.; Tamura, K., 2015. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0. Molecular Biology and Evolution. www.kumarlab.net/ publications.#
27. Tsukidate, J., 1984. Studies on the regenerative ability of the brown algae, Sargassum muticum (Yendo) Fensholt and Sargassum tortile C. Agardh. Hydrobiologia, 116 (117): 393-397.#
28. Yoshida, T.; Stiger, V.; Horiguchi, T., 2000. Sargassum boreale sp. nov. (Fucales, Phaeophyceae) from Hokkaido, Japan. Phycology Research Journal, 48: 125-131.#
29. دادالهی، س، گراوند کریمی، م، عماد آبادی، ا، 1391. بررسی تغییرات فصلی پراکنش و میزان زی توده جلبکهای غالب سواحل جزر و مدی استان بوشهر (ساحل شمالی خلیج فارس). نشریه اقیانوس-شناسی. شماره 9، صفحات 26-17.#
30. دادالهی، س، گراوند کریمی، م، عماد آبادی، ا، 1391. بررسی تغییرات فصلی پراکنش و میزان زی توده جلبکهای غالب سواحل جزر و مدی استان بوشهر (ساحل شمالی خلیج فارس). نشریه اقیانوس-شناسی. شماره 9، صفحات 26-17.#
31. Abbas, A.; Shameel, M., 2009. Anatomical studies on Colpomenla sinuous (Phaeophycota) Fromkarachi coast of Pakistan. Pakistan Journal of Botany, 41: 1921-1926.#
32. Abbas, A.; Shameel, M., 2014. Morpho-Anatomy of Styopodium zonale (Phaeophyta) from the coast of karachi. Pakistan Journal of Botany, 46: 1495-1499.#
33. Andrade-Sorcia, G.; Riosmena-Rodríguez, R.; Mu-iz-Salazar, R.; López-Vivas, J.M.; Boo, G.H.; Lee, K.M.; Boo, S.M., 2014. Morphological reassessment and molecular assessment of Sargassum (Fucales: Phaeophyceae) species from the Gulf of California, México. Phytotaxa, 183: 201-223.# [DOI:10.11646/phytotaxa.183.4.1]
34. Borgesen, F., 1939. Marine algae from the Persian Gulf especially from the innermost part near Bushire and the Island Kharg. In: Jessen K, Spärck R (eds) Danish Scientific Investigations in Iran, Part I. Copenhagen, 47–141PP.#
35. Bitner, L.; Payri, C.E.; Couloux, A.; Cruaud, C.; De Reviers B.; Rousseau, F., 2008. Molecular phylogeny of the Dictyotales and their position within the Phaeophyceae based on nuclear, plastid and mitochondrial DNA sequence data. Molecular Phylogenetic and Evolution Journal, 49: 211-226.# [DOI:10.1016/j.ympev.2008.06.018]
36. Baldock, R.N., 2009. Pictured key to the southern Australian species of Sargassum Algae Revealed. Australian State Herbarium, Australia, 140P.#
37. Bast, F., 2014. Sequence similarity search, multiple sequence alignment, model selection, distance matrix and phylogeny reconstruction. Nature Protocol Exchange, 1: 1-5.#
38. Cho, G.Y.; Rousseau, F.; De Reviers, B.; Boo, S.M., 2006. Phylogenetic relationships within the Fucales (Phaeophyceae) assessed by the photosystem I coding psaA sequences. Phycologia, 45: 512-519.# [DOI:10.2216/05-48.1]
39. Coyer, A.; Hoarau, G.; Oudot–Le Secq, M.; StamW.T.; Olsen, J.L., 2006. A mtDNA – based phylogeny of the brown algal genus Fucus (Heterokontophyta; Phaeophyta). Molecular Phylogenetics and Evolution Journal, 39: 209-222.# [DOI:10.1016/j.ympev.2006.01.019]
40. Cho, S.M.; Lee, S.M.; KO, Y.D.; Mattio, L.; Boo, S.M., 2012. Molecular systematic reassessment of Sargassum (Fucales, Phaeophyceae) in Korea using four gene regions. Botanica Marina, 55: 473-484.# [DOI:10.1515/bot-2012-0109]
41. Camacho, O.; Mattio, L.; Draisma, S.; Fredericq, S.; Diaz- Pulido, G., 2015. Morphological and molecular assessment of Sargassum (Fucales, Phaeophyceae) from Caribbean Colombia, including the proposal of Sargassum giganteum sp. nov., Sargassum schnetteri comb. Nov and Sargassum section Cladophyllum sect. nov. Systematics and Biodiversity, 13: 105-130.# [DOI:10.1080/14772000.2014.972478]
42. Draisma, S.G.A.; Baliesteros, E.; Rousseau, F.; Thibaut, T., 2010. DNA sequence data demonstrate the polyphyly of the genus Cystoseira and other Sargassaceae genera (Phaeophyceae). Journal of Phycology, 46: 1329-1345.# [DOI:10.1111/j.1529-8817.2010.00891.x]
43. Dixon, R.R.M.; Huisman, J.M.; Buchanan, J.; Gurgel, C.F.D.; Spencer, P., 2012. A morphological and molecular study of austral Sargassum (Fucales, phaeophyceae) supports the recognition of phyllotricha at genus level, with further additions to the genus Sargassopsais. Journal of Phycology, 48: 1119-1129.# [DOI:10.1111/j.1529-8817.2012.01187.x]
44. Garretta, A., 2000. Cystoseira C. Agardh. In: A. Gomez Garreta (Editor). Flora phycologica Iberica, Vol.1 Fucales. University of Murcia, Publications Services, Barcelona, 99-101P.#
45. Guiry, M.D.; Guiry, G.M., 2017. Algae Base. Galway: World-wide electronic publication, National University of Ireland. http://www.algaebase.org, accessed 30 September 2014.#
46. Hurtado, A.Q.; Ragaza, A.R., 1999. Sargassum studies in Currimao, Ilocos Norte, Northern Philippines I. Seasonal variations in the biomass of Sargassum carpophyllum J. Agardh, Sargassum ilicifolium (Turner) C. Agardh and Sargassum siliquosum J. Agardh (Phaeophyta, Sargassaceae). Botanica Marina, 42(4), 321-325.# [DOI:10.1515/BOT.1999.036]
47. Jegou, C.; Culoili, G.; Kervarek, N.; Simon, G.; Stiger-Pouvreau, V., 2010. LC/ESI-MS and 1H HR-MAS NMR analytical methods as useful taxonomical tools within the genus Cystoseira C. Agardh (Fucales; Phaeophyceae). Talanta, 83: 613-622.# [DOI:10.1016/j.talanta.2010.10.003]
48. Kurihara, A.; West, J.A.; Conklin, K.Y.; Sherwood, A.R., 2012. A second species of Rhodochlya from Hawaii, with a description of R. hawaiiana cryptogamie, Algology, 33: 21-33.#
49. Mattio, L.; Payri, C.E., 2011. 190 years of Sargassum Taxonomy, Facing the Advent of DNA Phylogenies. The Botanical Review, 77: 31-70.# [DOI:10.1007/s12229-010-9060-x]
50. Phillips, N.; Fredericq, S., 2000. Biogeographic and phylogenetic investigations of the pantropical genus Sargassum (Fucales, Phaeophyceae) with respect to Gulf of Mexico Species. Gulf of Mexico Science, 2: 77-87.#
51. Richmond, R.H.P.; Houk, M.; Trianni, E.; Wolanski, G.; Davis,V.; Paul, V.J., 2008. Aspects of biology and ecology functioning of coral reefs in Guam and the Commonwealth of the Northern Mariana Island: Riegl, B.M., Dodge, R.E. (eds.), coral reefs of the word. Springer, 719-739P.#
52. Sohrabipour, J.; Rabii, R., 1999. A list of marine algae of seashores of Persian Gulf and Oman Sea in the Hormozgan Province. Iranian Journal of Botany, 8: 131-162.#
53. Stiger, V.; Horiguchi, T.; Yoshida, T.; Coleman, A.W.; Masuda, M., 2003. Phylogenetic relationships inferred from ITS-2 nDNA comparisons within the genus Sargassum (Fucales, Phaeophyceae) from the Pacific basin, with an emphasis on the taxonomic subdivision of the genus. Phycological Research, 51: 1-10.# [DOI:10.1111/j.1440-1835.2003.tb00164.x]
54. Shams, M.; Afsharzadeh, S.; Balali, Gh.; De Clerck, O., 2013. Revision Sargassum species (Fucals, Phaeophyceae) from Persian Gulf and Oman Sea (Iran) based on morphological and phylogenetical analyses. Proceeding of 18th National Symposium on Applied Biological Sciences. February. 27-30, 2013. Ghent University, Ghent, Belgium, 253P.#
55. Shams, M.; Afsharzadeh, S.; Balali, Gh., 2015. Taxonomic Study of Six Sargassum Species (Sargassaceae, Fucales) with Compressed Primary Branches in the Persian Gulf and Oman Sea Including S. binderi Sonder a New Record Species for Algal Flora, Iran. Journal of Sciences, Islamic Republic of Iran, 26: 7-16.#
56. Kumar, S.; Tamura, K., 2015. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0. Molecular Biology and Evolution. www.kumarlab.net/ publications.#
57. Yoshida, T.; Stiger, V.; Horiguchi, T., 2000. Sargassum boreale sp. nov. (Fucales, Phaeophyceae) from Hokkaido, Japan. Phycology Research Journal, 48: 125-131.# [DOI:10.1111/j.1440-1835.2000.tb00207.x]
58. Abbas, A.; Shameel, M., 2009. Anatomical studies on Colpomenla sinuous (Phaeophycota) Fromkarachi coast of Pakistan. Pakistan Journal of Botany, 41: 1921-1926.#
59. Abbas, A.; Shameel, M., 2014. Morpho-Anatomy of Styopodium zonale (Phaeophyta) from the coast of karachi. Pakistan Journal of Botany, 46: 1495-1499.#
60. Andrade-Sorcia, G.; Riosmena-Rodríguez, R.; Mu-iz-Salazar, R.; López-Vivas, J.M.; Boo, G.H.; Lee, K.M.; Boo, S.M., 2014. Morphological reassessment and molecular assessment of Sargassum (Fucales: Phaeophyceae) species from the Gulf of California, México. Phytotaxa, 183: 201-223.# [DOI:10.11646/phytotaxa.183.4.1]
61. Borgesen, F., 1939. Marine algae from the Persian Gulf especially from the innermost part near Bushire and the Island Kharg. In: Jessen K, Spärck R (eds) Danish Scientific Investigations in Iran, Part I. Copenhagen, 47–141PP.#
62. Bitner, L.; Payri, C.E.; Couloux, A.; Cruaud, C.; De Reviers B.; Rousseau, F., 2008. Molecular phylogeny of the Dictyotales and their position within the Phaeophyceae based on nuclear, plastid and mitochondrial DNA sequence data. Molecular Phylogenetic and Evolution Journal, 49: 211-226.# [DOI:10.1016/j.ympev.2008.06.018]
63. Baldock, R.N., 2009. Pictured key to the southern Australian species of Sargassum Algae Revealed. Australian State Herbarium, Australia, 140P.#
64. Bast, F., 2014. Sequence similarity search, multiple sequence alignment, model selection, distance matrix and phylogeny reconstruction. Nature Protocol Exchange, 1: 1-5.#
65. Cho, G.Y.; Rousseau, F.; De Reviers, B.; Boo, S.M., 2006. Phylogenetic relationships within the Fucales (Phaeophyceae) assessed by the photosystem I coding psaA sequences. Phycologia, 45: 512-519.# [DOI:10.2216/05-48.1]
66. Coyer, A.; Hoarau, G.; Oudot–Le Secq, M.; StamW.T.; Olsen, J.L., 2006. A mtDNA – based phylogeny of the brown algal genus Fucus (Heterokontophyta; Phaeophyta). Molecular Phylogenetics and Evolution Journal, 39: 209-222.# [DOI:10.1016/j.ympev.2006.01.019]
67. Cho, S.M.; Lee, S.M.; KO, Y.D.; Mattio, L.; Boo, S.M., 2012. Molecular systematic reassessment of Sargassum (Fucales, Phaeophyceae) in Korea using four gene regions. Botanica Marina, 55: 473-484.# [DOI:10.1515/bot-2012-0109]
68. Camacho, O.; Mattio, L.; Draisma, S.; Fredericq, S.; Diaz- Pulido, G., 2015. Morphological and molecular assessment of Sargassum (Fucales, Phaeophyceae) from Caribbean Colombia, including the proposal of Sargassum giganteum sp. nov., Sargassum schnetteri comb. Nov and Sargassum section Cladophyllum sect. nov. Systematics and Biodiversity, 13: 105-130.# [DOI:10.1080/14772000.2014.972478]
69. Draisma, S.G.A.; Baliesteros, E.; Rousseau, F.; Thibaut, T., 2010. DNA sequence data demonstrate the polyphyly of the genus Cystoseira and other Sargassaceae genera (Phaeophyceae). Journal of Phycology, 46: 1329-1345.# [DOI:10.1111/j.1529-8817.2010.00891.x]
70. Dixon, R.R.M.; Huisman, J.M.; Buchanan, J.; Gurgel, C.F.D.; Spencer, P., 2012. A morphological and molecular study of austral Sargassum (Fucales, phaeophyceae) supports the recognition of phyllotricha at genus level, with further additions to the genus Sargassopsais. Journal of Phycology, 48: 1119-1129.# [DOI:10.1111/j.1529-8817.2012.01187.x]
71. Garretta, A., 2000. Cystoseira C. Agardh. In: A. Gomez Garreta (Editor). Flora phycologica Iberica, Vol.1 Fucales. University of Murcia, Publications Services, Barcelona, 99-101P.#
72. Guiry, M.D.; Guiry, G.M., 2017. Algae Base. Galway: World-wide electronic publication, National University of Ireland. http://www.algaebase.org, accessed 30 September 2014.#
73. Hurtado, A.Q.; Ragaza, A.R., 1999. Sargassum studies in Currimao, Ilocos Norte, Northern Philippines I. Seasonal variations in the biomass of Sargassum carpophyllum J. Agardh, Sargassum ilicifolium (Turner) C. Agardh and Sargassum siliquosum J. Agardh (Phaeophyta, Sargassaceae). Botanica Marina, 42(4), 321-325.# [DOI:10.1515/BOT.1999.036]
74. Jegou, C.; Culoili, G.; Kervarek, N.; Simon, G.; Stiger-Pouvreau, V., 2010. LC/ESI-MS and 1H HR-MAS NMR analytical methods as useful taxonomical tools within the genus Cystoseira C. Agardh (Fucales; Phaeophyceae). Talanta, 83: 613-622.# [DOI:10.1016/j.talanta.2010.10.003]
75. Kurihara, A.; West, J.A.; Conklin, K.Y.; Sherwood, A.R., 2012. A second species of Rhodochlya from Hawaii, with a description of R. hawaiiana cryptogamie, Algology, 33: 21-33.#
76. Mattio, L.; Payri, C.E., 2011. 190 years of Sargassum Taxonomy, Facing the Advent of DNA Phylogenies. The Botanical Review, 77: 31-70.# [DOI:10.1007/s12229-010-9060-x]
77. Phillips, N.; Fredericq, S., 2000. Biogeographic and phylogenetic investigations of the pantropical genus Sargassum (Fucales, Phaeophyceae) with respect to Gulf of Mexico Species. Gulf of Mexico Science, 2: 77-87.#
78. Richmond, R.H.P.; Houk, M.; Trianni, E.; Wolanski, G.; Davis,V.; Paul, V.J., 2008. Aspects of biology and ecology functioning of coral reefs in Guam and the Commonwealth of the Northern Mariana Island: Riegl, B.M., Dodge, R.E. (eds.), coral reefs of the word. Springer, 719-739P.#
79. Sohrabipour, J.; Rabii, R., 1999. A list of marine algae of seashores of Persian Gulf and Oman Sea in the Hormozgan Province. Iranian Journal of Botany, 8: 131-162.#
80. Stiger, V.; Horiguchi, T.; Yoshida, T.; Coleman, A.W.; Masuda, M., 2003. Phylogenetic relationships inferred from ITS-2 nDNA comparisons within the genus Sargassum (Fucales, Phaeophyceae) from the Pacific basin, with an emphasis on the taxonomic subdivision of the genus. Phycological Research, 51: 1-10.# [DOI:10.1111/j.1440-1835.2003.tb00164.x]
81. Shams, M.; Afsharzadeh, S.; Balali, Gh.; De Clerck, O., 2013. Revision Sargassum species (Fucals, Phaeophyceae) from Persian Gulf and Oman Sea (Iran) based on morphological and phylogenetical analyses. Proceeding of 18th National Symposium on Applied Biological Sciences. February. 27-30, 2013. Ghent University, Ghent, Belgium, 253P.#
82. Shams, M.; Afsharzadeh, S.; Balali, Gh., 2015. Taxonomic Study of Six Sargassum Species (Sargassaceae, Fucales) with Compressed Primary Branches in the Persian Gulf and Oman Sea Including S. binderi Sonder a New Record Species for Algal Flora, Iran. Journal of Sciences, Islamic Republic of Iran, 26: 7-16.#
83. Kumar, S.; Tamura, K., 2015. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0. Molecular Biology and Evolution. www.kumarlab.net/ publications.#

XML   Persian Abstract   Print

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 8, Issue 31 (2017) Back to browse issues page