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:: Volume 10, Issue 38 (2019) ::
2019, 10(38): 45-54 Back to browse issues page
Comparative immunohistochemistry of Gut-associated lymphoid tissue (GALT) of, Huso huso in two warm and cold seasons
Abstract:   (4284 Views)
Abstract
The main objective of recent study was comparative immunohistochemistry of Gut-associated lymphoid tissue (GALT) of, Huso huso in two warm and cold seasons. Ten specimens were collected from sturgeon breeding centers and after anesthesia, biometric was taken on them. 0.5 centimeter samples of primary, middle and end of intestine were removed and immediately were placed in Bouin solution. Rutine procedure of tissue prepration was done and sections in the thicknesses of 4-6 microns were prepared. Eventually they were taken under staining. Finally was performed general Hematoxylin-Eosin staining (H&E), histochemical staining of Periodic Acid Schiff (PAS), and (TUNEL) immunohistochemistry. Histomorphology and histometric results showed that the intestine consists of mucous, submucosa, muscularis and serousa. Comparative study of Gut-associated lymphoid tissue in two seasons was found that their density and composition are different. In cold season, the density of lymphoid tissue in base of the epithelium was more than the warm season.The number of lymphocyte cells in the mucosa and submucosa of different parts of the intestine in warm season showed a significant decrease compare to the cold season (p≤0.05). Also, the number of lymphocyte cells in the end of the intestine showed a significant increase during the cold season compare to the warm season (p≤0.05). In general, it has been shown that in immunohistochemical studies, the number of cells with apoptosis, especially in the warm season, were higher in all parts of the intestine than to the cold season (p≥0.05). Therefore, due to stimulation immune system in this part of the gastrointestinal tract, it can be considered to determine for vaccination time.
Keywords: Immunohistochemistry, GALT, Intestine, Huso huso.
Full-Text [PDF 592 kb]   (1456 Downloads)    
Type of Study: Research/ Original/ Regular Article | Subject: Marine Biology
Received: 2018/02/16 | Revised: 2021/05/12 | Accepted: 2018/09/17 | ePublished: 2020/05/12
References
1. Abdulmanafi, A.; Liem, P.; Van, M.; Ambak, M., 2006. Histological ontogeny of the digestive system of Marble goby (Oxyeleotris marmoratus) Larvae. Journal of Sustainability and Management Science, 2: 79-86.
2. Ahmadifar, E.; Falahatkar, B.; Akrami, R., 2011. Effects of dietary thymol-carvacrol on growth performance, hematological parameters and tissue composition of juvenile rainbow trout, Oncorhynchus mykiss. Journal of Applied Ichthyology, 1: 1-4. [DOI:10.1111/j.1439-0426.2011.01763.x]
3. Ahmed, Y.; El-Hafez, A.A.; Zayed, A.E., 2009. Histological and Histochemical Studies on the Esophagus, Stomach and Small Intestines of Varanus niloticus. Journal of veterinary anatomy, 2: 35-48. [DOI:10.21608/jva.2009.45136]
4. Banankhojasteh, S.; Sheykhzadeh, F.; Mohammadnejad, D.; Azami, A., 2009. Histological, histochemical and ultrastructrual study of the intestine of rainbow trout (Oncorhynchus mykiss). Journal of World Applied Science, 6:1525-1531.
5. Bastos, R.; Miranda, S.F. ; Garcia, A. M., 2013. Dieta e estrategia alimentar de Characidium rachovii (Characiformes, Crenuchidae) em riachos de planicie costeira do sul do Brasil. Iheringia Serie Zoologia, 103: 335-341 [DOI:10.1590/S0073-47212013000400001]
6. Bhaskara, C.; Wallace, S.N.; Naisandra, B.S. ; Sathyabama, C., 2012. Morphohistology of the Digestive Tract of the Damsel Fish (Stegastes fuscus) (Osteichthyes: Pomacentridae). Journal Scientific World, 13:78-82. [DOI:10.1100/2012/787316]
7. Bocina, Z.; Santic, I.; Restovi, c.; Topi, S.C., 2017. Histology of the digestive system of the garfish Belone belone (Teleostei: Belonidae). The European Zoological Journal, 84: 89-95 [DOI:10.1080/11250003.2016.1276977]
8. Boncroft, J.; Gamble, M., 2002. Theory and practice of histological Techniques. Five edition. Churchil Living stone, London, pp:152-155.
9. Campoverde, C.; Andree, KB.; Milne, DJ.; Estevez, A.; Gisbert, E.; Carella, F., 2018. Ontogeny of lymphoid organs and mucosal associated lymphoid tissues in meagre (Argyrosomus regius). Fish Shellfish Immunol, 15(48): 509-520. [DOI:10.1016/j.fsi.2018.09.033]
10. Canan, B.; Nascimento, W.S.D.; Silva, N.B.D.; Chellappa, S., 2012. Morphohistology of the digestive tract of the damsel fish Stegastes Fuscus (Osteichthyes: Pomacentridae). The Scientific World Journal, 12:1-9. [DOI:10.1100/2012/787316]
11. Coteur, G.; Corriere, N.; Dubois, P.h., 2004. Environmental factors influencing the immune responses of the common european starfish (Asterios rubens). Fish and Sellfish immunology, 16: 51-55. [DOI:10.1016/S1050-4648(03)00030-5]
12. Dai, X. ; Shu, M.; Fang, W., 2007. Histological and ultra-structural study of the digestive tract of rice field eel (Monopterus albus). Journal Applied Ichthyology, 23: 177-183. [DOI:10.1111/j.1439-0426.2006.00830.x]
13. Day, R.D.; Tibbetts, I.R.; Secor, S.M., 2014. Physiological responses to short-term fasting among herbivorous, omnivorous, and carnivorous fishes. Journal of Comparative Physiology B,184: 497-512. [DOI:10.1007/s00360-014-0813-4]
14. Dellman, H., 2006. Veterinary histology. An outline text-atlas.sixth edition. LEA& febiger. Philadelphia, pp: 137-169.
15. Duncan, N.J.; Estevez, A.; Gairin, I.; Schuchardt, R., 2013. Aquaculture production of meagre (Argyrosomus regius): hatchery techniques, ongrowing and market Advanced Aquaculture Hatchery Technology, 32: 519-541. [DOI:10.1533/9780857097460.3.519]
16. Estensoro, I.; Jung-Schroers, P.; Steinhagen, A., 2013. Effects of Enteromyxum leei (Myxozoa) infection on gilthead sea bream (Sparus aurata) (Teleostei) intestinal mucus: glycoprotein profile and bacterial adhesion. [DOI:10.1007/s00436-012-3168-3]
17. Fish Shellfish Immunol, 35:1729-1739
18. Germano, R.M.; Stabille, S.R.; Mari, R.B., 2014. Morphological characteristics of the Pterodoras granulosus digestive tube (Valenciennes, 1821) Acta Zoologica (Osteichthyes, Doradidae), 95:166-175. [DOI:10.1111/azo.12016]
19. Gomez, D.; Sunyer, I., 2013. The mucosal immune system of fish: the evolution of tolerating commensals while fighting pathogens. Parasitology Reserch, 112: 567-576.
20. Horvat, L.; Tomos, G.; Cycro, K., 2005. Upbringing of Carp and other farmed fish. Translate by: Khoshkholgh MR. Gilan univ press, pp: 77-79.
21. John, E.; Ronald, W., 2002. Fish nutrition. thired edition. Academic press inc. pp: 234-237, 340-346.
22. Khoshkholgh, M.R.; Nazari, S.B.; Pourkazemi, M.C., 2013. Population structure of Persian sturgeon (Acipenser persicus Borodin, 1897) in the southern part of Caspian Sea. Iranian Journal of Animal Biosystematics, 9(1): 29-39.
23. Kuperman, B.; Kuzmian, V., 1994. The ultrastruature of the intestinal epithelium in fishes with different types of feeding, Journal of Fish Biology, 44: 181-193. [DOI:10.1111/j.1095-8649.1994.tb01197.x]
24. Nikoskelainen, S.; Goran, B.; Lilius, E., 2004. Effect of environmental temperature on rainbow trout (Oncorhynchus mykiss) innate immunity. Developmental & Comparative Immunology, 28(6): 581-92. [DOI:10.1016/j.dci.2003.10.003]
25. Papadakis, I.E.; Kentouri, M.; Divanach, P.; Mylonas, C.C. 2016. Ontogeny of the digestive system of meagre Argyrosomus regius reared in a mesocosm, and quantitative changes of lipids in the liver from hatching to juvenile. Aquaculture, 433: 360-366.
26. Pogoreutz, C.; Ahnelt, H., 2014. Gut morphology and relative gut length do not reliably reflect trophic level in gobiids: a comparison of four species from a tropical Indo-Pacific seagrass bed. Journal of Applied Ichthyology, 30: 408-410. [DOI:10.1111/jai.12295]
27. Pousti, I., 2005. Comprative histology and histotechnique. Third Edition. Tehran University Press.pp: 167-168.
28. Raji, A.R. ; Norouzi, E., 2010. Histological and histochemical study on the alimentary canal in walking catfish (Claris batrachus) and Piranha (Serrasalmus nattereri). Iranian Journal of Veterinary Research, 11: 255-261.
29. Rodrigues, M.; Marcal, M.; Segatti, H., 2012. Histology of the digestive tract of satanoperca pappaterra (Osteichthyes, Cichlidae). Journal of Acta Scientiarum, 34: 319-326. [DOI:10.4025/actascibiolsci.v34i3.8956]
30. Santos, M.; Arantes, F.; Pessali, T., 2015. Morphological, histological and histochemical analysis of the digestive tract of Trachelyopterus striatulus (Siluri- formes: Auchenipteridae). Zoologica (Curitiba), 32: 296-30 [DOI:10.1590/S1984-46702015000400005]
31. Solovyev, M.; Campoverde, S.; Moreira, M., 2016. Morphological and functional description of the development of the digestive system in meagre (Argyrosomus regius): an integrative approach. Aquaculture, 464: 381-391. [DOI:10.1016/j.aquaculture.2016.07.008]



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Comparative immunohistochemistry of Gut-associated lymphoid tissue (GALT) of, Huso huso in two warm and cold seasons. Journal of Oceanography 2019; 10 (38) :45-54
URL: http://joc.inio.ac.ir/article-1-1513-en.html


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Volume 10, Issue 38 (2019) Back to browse issues page
نشریه علمی پژوهشی اقیانوس شناسی Journal of Oceanography
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